The novel cyclophilin inhibitor C105SR reduces hepatic ischaemia–reperfusion injury via mitoprotection
Résumé
Study of the ability to inhibit CypD peptidyl-prolyl cis-trans isomerase (PPIase) activity and of mitoprotective properties
CypD PPIase activity assay
Mitochondrial swelling assay
Mitochondrial calcium retention capacity assay
Isolated mouse liver mitochondria 1 compound
Study of the ability to inhibit mitochondrial permeability transition pore opening and cell death
In vitro hypoxia/reoxygenation model
Mouse hepatocyte cell line
Study of the hepatoprotective properties
Identification of credible lead compound for subsequent clinical development
In vivo hepatic ischemia/reperfusion injury model
compounds
Cyclophilin inhibitor synthesis
Chemical optimization R1
Mots clés
Cellular protection
Peptidyl-prolyl cis-trans isomerase activity
Liver necrosis
Mitochondrial calcium retention capacity
Mitochondrial permeability transition pore
Mitochondrial swelling
Cellular protection Peptidyl-prolyl cis-trans isomerase activity Liver necrosis Mitochondrial calcium retention capacity Mitochondrial permeability transition pore Mitochondrial swelling
Domaines
Sciences du Vivant [q-bio]| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
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