Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing - Institut Mondor de Recherche Biomédicale
Article Dans Une Revue Communications Biology Année : 2022

Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing

Résumé

The ocular vasculature is critically involved in many blinding diseases and is also a popular research model for the exploration of developmental and pathological angiogenesis. The development of ocular vessels is a complex, finely orchestrated sequence of events, involving spatial and temporal coordination of hyaloid, choroidal and retinal networks. Comprehensive studies of the tridimensional dynamics of microvascular remodeling are limited by the fact that preserving the spatial disposition of ocular vascular networks is cumbersome using classical histological procedures. Here, we demonstrate that light-sheet fluorescence microscopy (LFSM) of cleared mouse eyes followed by extensive virtual dissection offers a solution to this problem. To the best of our knowledge, this is the first 3D quantification of the evolution of the hyaloid vasculature and of post-occlusive venous remodeling together with the characterization of spatial distribution of various cell populations in ocular compartments, including the vitreous. These techniques will prove interesting to obtain other insights in scientific questions addressing organ-wide cell interactions.
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hal-04863875 , version 1 (04-01-2025)

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Marie Darche, Anna Verschueren, Morgane Belle, Leyna Boucherit, Stéphane Fouquet, et al.. Three-dimensional characterization of developing and adult ocular vasculature in mice using in toto clearing. Communications Biology, 2022, 5 (1), pp.1135. ⟨10.1038/s42003-022-04104-2⟩. ⟨hal-04863875⟩
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