Viral infections of the nervous system are often very serious affections, and can be responsible for fatal encephalitis or severely disabling neurological sequelae. In humans, many viruses are capable of infecting the nervous system; some of them are also responsible for encephalitis in horses. However, there are currently very few antiviral treatments available, and none to block the replication of neurotropic arboviruses (viruses transmitted by mosquitoes and ticks) and limit the damage caused to the brain. Our aim is to identify broad-spectrum antivirals that will be effective against several viral families, in both humans and horses. This poster will show the different infection models based on 2D/3D cultures of central nervous system cells that we have developed in recent years, and our strategy for identifying broad-spectrum antiviral compounds active in the nervous system.